Our Technology - TZX4

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TZX4 is a culture medium  developed by Dr.  Shinn-Zhong Lin and his team at the Hualien Tzu Chi Hospital.  Through a technology transfer partnership, we are applying this technology towards stem cell treatments to deliver higher efficacy solutions to patients.

TZX4是由Shinn ZhongLin博士開發的培養基（ 林欣榮院長)  和他的團隊在花蓮慈濟醫院。 通過技術轉移合作夥伴關係，我們正在將這項科技應用於幹細胞治療，為患者提供更高效的解決方案。

Partial Reprogramming Helps Maintain Cells at Their Prime Stage

部分重新程式設計有助於維持細胞處於最佳階段

Donor age is a major factor in stem cell efficacy, with older aged cells negatively impacting efficacy. ADSCs isolated from elderly donors have decreased biological activity, including differentiation and regenerative potential. The introduction of TZX4 in the culture medium has shown the ability to restore cells to their prime states – for example in  studies, cells from a 64-year old patient were restored to a 34-year old state after 11 days. After 15 days they were restored to the equivalent of a 19-year old state

供體年齡是影響幹細胞療效的主要因素，年齡較大的細胞會對療效產生負面影響。 從老年供體中分離的ADSCs具有降低的生物活性，包括分化和再生潜力。 TZX4在培養基中的引入表明了將細胞恢復到原始狀態的能力——例如，在我們研究團隊進行的研究中，一名64歲患者的細胞在11天后恢復到34歲的狀態。 15天后，它們恢復到相當於19年前的狀態

Adipose Derived Stem Cell (ADSC) Growth 脂肪來源幹細胞（ADSC）生長

As stem cells age, their renewal ability deteriorates. TZX4 culture medium has shown its ability to facilitate rapid ADSC growth 

隨著幹細胞的衰老，它們的更新能力會下降。 TZX4培養基已顯示出促進ADSC快速生長的能力

Maintaining Gene Expression of OCT4 and Nanog 維持OCT4和Nanog的基因表達

Oct4 and Nanog are key regulatory genes that maintain the pluripotency and self-renewal properties of stem cells. The TZX4 medium helps the rapid growth of ADSC and expression of these key stem cell genes compared to other methods. After optimization, the expression of stemness genes in "old adult stem cells" increased by 1.5 times

Oct4和Nanog是維持幹細胞多能性和自我更新特性的關鍵調控基因。 與其他方法相比，TZX4培養基有助於ADSC的快速生長和這些關鍵幹細胞基因的表達。 優化後，幹性基因在“老年幹細胞”中的表達新增了1.5倍

Maintaining Differentiation Properties 保持差异化内容

TZX4 medium-cultured ADSC has the ability to differentiate into adipocyte, osteocyte, and chondrocyte.

TZX4培養基培養的ADSC具有分化為脂肪細胞、骨細胞和軟骨細胞的能力。

Reduction of  Cell Inflammation 减少細胞炎症

Reducing cell inflammation is the key to cell growth; when cells are damaged, TZX4 can reduce cell inflammatory cytokines, e.g., IL-6、IL-8、MIF.

减少細胞炎症是細胞生長的關鍵； 當細胞受損時，TZX4可以降低細胞炎症細胞因數，如IL-6、IL-8、MIF。

TZX4-cultured ADSC Secretes Significantly Greater Amounts of Exosomes

TZX4培養的ADSC分泌大量外泌體